Traceability of Sicilian Durum Wheat Landraces and Historical Varieties by High Molecular Weight Glutenins Footprint

Over the last new decade, there has been a strong interest in landraces and historical wheat varieties from farmers, manufacturers consumers. They are agronomically nutritionally interesting but supply chain (from seed to end-product) is not solid traceable. High molecular weight glutenins (HMW-GS) can act as markers trace varietal correspondence verify genetic purity of grain consequently flours, marketed labeled mono-varietal. In present work, HMW-GS different durum Sicilian (Timilia, Russello, Perciasacchi) one variety Margherito were analyzed. At first, specific protein profiles assigned each landrace by SDS-PAGE MALDI-TOF/MS analyses, thanks availability pure seeds. Analysis then carried out random samples batches same grown on farm South-East Sicily. The results highlighted presence bands within individual batches, which reflected complex corresponding commercial flours labelled bread Maiorca cultivated was also found contaminant at percentages batches. this study offer opportunities improve or cultivated, underlining need for accurate controls field transformation process be mono-varietal products.